BSA-Block saturates free binding sites on plastic surfaces or other protein binding surfaces for minimizing non-specific binding to surfaces.
BSA-Block is a well established, versatile blocker for many applications.
The high purity and control of the raw materials enable good lot-to-lot consistency for this blocker based on BSA. Therefore a high reliability of the assay over years is given even when different lots are used.
If you experience background due to non-specific binding even when using BSA-Block, we recommend using The Blocking Solution.
Albumins form a large fraction of the matrix where antibodies can be naturally found.Some antibodies need the presence of albumins in their direct surrounding to achieve optimal binding characteristics. In case of using an albumin-sensitive capture antibody it makes sense to use BSA-Block as blocking reagent.
During blocking with BSA-Block, albumin molecules adsorb closely to the coated capture antibodies und enable good binding of the antibodies. This can be observed in a better binding capacity of coated capture antibodies blocked with BSA-Block compared to blocking with other blockers. It is recommended to test different blockers in parallel when assembling a new assay because the previously described effect occurs rarely.
The blocking efficiency of innovative blockers such as SmartBlock™ or The Blocking Solution is superior to classic BSA blockers and can be used if the capture antibodies is less dependent on BSA. Currently no efficient buffer alternatives exist for BSA sensitive antibodies.
For BSA insensitive antibodies, very efficient and economic blocking solutions are available as substitutes.