LowCross-Buffer® used as an assay buffer helps you to improve signal to noise ratio.
What is specific to LowCross-Buffer®?
Why does LowCross-Buffer® reduce matrix effects?
The analyte can be masked by proteins or other components of the specimen. Thus the antibody can’t bind the analyte. Also antibodies can be masked by matrix components. LowCross-Buffer® reduces the masking of antibodies.
How do I have to use LowCross-Buffer®?
Do I have to dilute LowCross-Buffer®?
Sample or detection antibodies are diluted directly with the buffer. Only in competitive assays and some applications of immunohistochemistry it can be useful to dilute LowCross-Buffer® in water or physiological buffers. In this case the user has to find out the ideal mixing ratio by testing.
Can LowCross-Buffer® be used with monoclonal and polyclonal antibodies?
Can buffer components influence the colour reaction of enzymatic detection?
I get a high background in my sandwich ELISA which is detected by a peroxidase labelled secondary antibody. What can be the reason and can LowCross-Buffer® help?
Can I use LowCross Buffer® in combination with fluorescent dyes e.g. in protein arrays?
How often can I freeze and thaw LowCross-Buffer®?
Can LowCross-Buffer® replace a blocking solution for surfaces (e.g. Western blotting membranes, ELISA plates)?
No, not at all. LowCross-Buffer® is not a blocking buffer for surfaces. It is rather an assay buffer to be used for dilution of the specimens or antibodies. For blocking of surfaces such as membranes, we recommend using The Blocking Solution. This casein-based blocker is much more potent than most known blockers commercially available.