SmartBlock™ prevents non-specific and unwanted binding to surfaces, in many assays. By incubating with SmartBlock™, free spaces on the surface (of e.g. microtiter plate or western blotting membrane) are saturated by the components of SmartBlock™. Thus it prevents unwanted binding of the analyte or the detection antibody to the surface. As a result background can be avoided.
For many assays SmartBlock™ is an economical and useful alternative to BSA containing solutions.
Problems caused by BSA included in the blocker such as BSA-interactions and crossreactivities can be eliminated by using SmartBlock™.
The blocking efficiency depends on different aspects. The better the efficiency of the blocker, the more reliable are the results of the assays (even with different specimens). It is important to have a complete saturation of the surface with a dense and gap-free layer. Any interaction of molecules of the blocking layer with the sample matrix should also be prevented. A good blocker can even fill small gaps and should be designed to minimize interactions.
SmartBlock™ is a candidate of a new class of blockers developed by CANDOR Bioscience. These blockers have chemical modifications which help to minimize interactions between the blocking layer and the sample matrix.
SmartBlock™ is based on peptides which were chemically modified. The molecules of these blockers can fill small gaps on the blocked surface e.g. between close-fitting coated capture antibodies. This enables a sufficient blocking for many assays.
SmartBlock™ is an economical modern blocker with a high blocking efficiency.
The lot-to-lot consistency is very good. Thus SmartBlock™ is used even in the industrial production of immunodiagnostics.
To obtain an even better blocking effectivity compared to SmartBlock™, we recommend using The Blocking Solution. The Blocking Solution combines the advantages of blockers based on proteins with the good characteristics of modern chemically modified blockers. When reliability and very low coefficients of variation are important for your assay the use of The Blocking Solution is recommended.