Assay Defender® Cat. No. #180
Total HAMA and Interference Blocker
Ready-to use assay diluent for reduction of interference caused by HAMA (human anti-mouse antibodies), rheumatoid factors, nonspecific binding, cross-reactivities and matrix effects. Assay Defender® is particularly suitable for human or animal specimens containing HAMA (human anti-mouse antibodies), AAA (anti-animal antibodies) or other high affinity antibodies.
Assay Defender® can be used for different applications such as ELISA, lateral flow assays or bead-based assays. Examples are shown here.
For research use only, not for diagnostic use.
Interference in immunoassays can cause false positive and false negative results in routine testing.
Reasons for the increasing prevalence of different kinds of interference are for example:
- ageing population
- increasing number of patients with:
- chronic autoimmune diseases
- allergic and inflammatory diseases
- an improved limit of detection in modern assays
- more applications of sandwich-assay formats
Interferences can be classified due to their biochemical reasons and impact on assay performance.
- Interference caused by antibodies from patient samples
e.g. HAMA (human anti-mouse antibodies), HAAA (human anti-animal antibodies), heterophilic antibodies and rheumatoid factors from patient samples
- Interference caused by endogenous components of the sample
e.g. albumins, complement, lysozyme, fibrinogen, α-1 Antitrypsin, atypically high lipid-, salt- or sugar concentrations as well as atypical viscosities
- Interference caused by assay components
Assay components - like fluorescent or enzymatic labels - can cross-react with substances from the sample or change binding properties of the assay antibodies
In contrast to conventional HAMA blockers, Assay Defender® enables effective elimination of all kinds of interference and helps to prevent false positive or false negative results.
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Brand | CANDOR Bioscience |
Code | 180 125 |
Product Flyers and Application Guides
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Publications
CoViD-19-antibody-test-challenge
Interference and HAMA problems
IVD technology article
Long-term stabilization of assay components
Optimisation of assays: Interference in immunoassays recognize and avoid
SARS-CoV-2 antigen tests: safety for less than 5 cents per test
Specificity, positive predictive value and validation statistics in the context of CoViD-19
The appropriate blocker — A short overview
The right strategy saves much costs