Assay Defender® Cat. No. #180

Ready-to-use; Total HAMA and interference blocker. Assay Defender® is a ready-to use assay diluent for reduction of interference caused by HAMA (human anti-mouse antibodies), rheumatoid factors, nonspecific binding, cross-reactivities and matrix effects.
£118.62

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Total HAMA and Interference Blocker

Ready-to use assay diluent for reduction of interference caused by HAMA (human anti-mouse antibodies), rheumatoid factors, nonspecific bindingcross-reactivities and matrix effects. Assay Defender® is particularly suitable for human or animal specimens containing HAMA (human anti-mouse antibodies), AAA (anti-animal antibodies) or other high affinity antibodies.

Assay Defender® can be used for different applications such as ELISA, lateral flow assays or bead-based assays. Examples are shown here.

For research use only, not for diagnostic use.


Reasons for interference

Interference in immunoassays can cause false positive and false negative results in routine testing.

Reasons for the increasing prevalence of different kinds of interference are for example:

  • ageing population
  • increasing number of patients with:
    • chronic autoimmune diseases
    • allergic and inflammatory diseases
  • an improved limit of detection in modern assays
  • more applications of sandwich-assay formats

Interferences can be classified due to their biochemical reasons and impact on assay performance.

  1. Interference caused by antibodies from patient samples
    e.g. HAMA (human anti-mouse antibodies), HAAA (human anti-animal antibodies), heterophilic antibodies and rheumatoid factors from patient samples
     
  2. Interference caused by endogenous components of the sample
    e.g. albumins, complement, lysozyme, fibrinogen, α-1 Antitrypsin, atypically high lipid-, salt- or sugar concentrations as well as atypical viscosities
     
  3. Interference caused by assay components
    Assay components - like fluorescent or enzymatic labels - can cross-react with substances from the sample or change binding properties of the assay antibodies

In contrast to conventional HAMA blockers, Assay Defender® enables effective elimination of all kinds of interference and helps to prevent false positive or false negative results.

 

Download Product Resources

 Assay Defender® Use
 Assay Defender® Matrix

Brand CANDOR Bioscience
Code 180 125

Product Flyers and Application Guides

 BIOAXXESS CANDOR Blocker Selection Guide NEW
BIOAXXESS CANDOR Product Application Guide
 CANDOR Products Brochure
 Assay Defender® Flyer NEW
 Assay Defender® Use NEW
 Assay Defender® LowCross-Buffer® Comparison Matrix NEW
 SafetyTector™ S NEW
 


Product Inserts and Data Sheets

Assay Optimizers

 Assay Defender® Cat. No. #180  NEW
 LowCross-Buffer® Cat. No. #100, #101, #102
 LowCross-Buffer® Animal-free/Protein-free Cat. No. #300, #301, #302
 Low-Cross-HRP® Cat. No. #200
 LowCross® HRP-Stab Cat. No. #270
 LowCross® HRP-Stab Animal-free/Protein-free Cat. No. #370
 Sample Buffer Cat. No. #105, #106
 Sample Buffer Animal-free/Protein-free Cat. No. #305


Covid-19 Inactivation and Dilution Buffer

 SafetyTector™ S Cat. No. #400  NEW
 

Blockers

 The Blocking Solution Cat. No. #110
 BSA-Block Cat. No. #115
 PlateBlock™ Cat. No. #112  NEW  
 SmartBlock™ Cat. No. #113


Stabilizers

 Antibody Stabilizer TRIS Cat. No. #130
 Antibody Stabilizer TRIS Animal-free/Protein-free Cat. No. #330
 Antibody Stabilizer PBS Cat. No. #131
 Antibody Stabilizer PBS Animal-free/Protein-free Cat. No. #331
 AP-Protector® Cat. No. #235
 Liquid Plate Sealer® Cat. No. #160
 Liquid Plate Sealer® Animal-free Cat. No. #163
 HRP-Protector™ Cat. No. #222
 HRP-Protector™ Animal-free/Protein-free Cat. No. #322


Standard Buffers (ready-made)

 Coating Buffer pH 7.4 Cat. No. #120
 Coating Buffer pH 9.6 Cat. No. #121
 Stripping Buffer Cat. No. #150
 Washing Buffer PBS Cat. No. #140
 Washing Buffer PBS IHC Cat. No. #141
 Washing Buffer TRIS Cat. No. #145
 Washing Buffer TRIS IHC Cat. No. #146


All-in-One Western Blotting Solution

 ReadyTector® Cat. No. #720 and 730


Tool boxes

 Blocking Sampler Package with BSA Block Cat. No. #600
 CANDOR Starter Package Cat. No. #600
 CANDOR Starter Package IHC Cat. No. #600


Publications

 CoViD-19-antibody-test-challenge
 Interference and HAMA problems
 IVD Technology Article
 Long-term stabilization of assay components
 Optimisation of assays: Interference in immunoassays recognize and avoid 
 SARS-CoV-2 antigen tests: safety for less than 5 cents per test 
 Specificity, positive predictive value and validation statistics in the context of CoViD-19
 The Appropriate Blocker — A Short Overview
 The right strategy saves much costs