The standard blocker for ELISA, EIA, Western blotting, protein arrays and immuno-PCR.
BSA-Block saturates free binding sites on plastic surfaces or other protein binding surfaces for minimizing non-specific binding to surfaces.
BSA-Block is a well established, versatile blocker for many applications.
The high purity and control of the raw materials enable good lot-to-lot consistency for this blocker based on BSA. Therefore a high reliability of the assay over years is given even when different lots are used.
If you experience background due to non-specific binding even when using BSA-Block, we recommend using The Blocking Solution.
Benefits of BSA blocking
Albumins form a large fraction of the matrix where antibodies can be naturally found.Some antibodies need the presence of albumins in their direct surrounding to achieve optimal binding characteristics. In case of using an albumin-sensitive capture antibody it makes sense to use BSA-Block as blocking reagent.
During blocking with BSA-Block, albumin molecules adsorb closely to the coated capture antibodies und enable good binding of the antibodies. This can be observed in a better binding capacity of coated capture antibodies blocked with BSA-Block compared to blocking with other blockers. It is recommended to test different blockers in parallel when assembling a new assay because the previously described effect occurs rarely.
The blocking efficiency of innovative blockers such as SmartBlock™ or The Blocking Solution is superior to classic BSA blockers and can be used if the capture antibodies is less dependent on BSA. Currently no efficient buffer alternatives exist for BSA sensitive antibodies.
For BSA insensitive antibodies, very efficient and economic blocking solutions are available as substitutes.
Download Product Resources
Product Descriptions & Guides
Product Inserts and Data Sheets
| Brand | CANDOR Bioscience |
| Code | 115 125 |
Product Flyers and Application Guides
Assay Optimizers
Assay Defender® Cat. No. #180
LowCross-Buffer® Cat. No. #100
LowCross-Buffer® Cat. No. #101, #102
LowCross-Buffer® Animal-free/Protein-free Cat. No. #300
Low-Cross-HRP® Cat. No. #200
LowCross® HRP-Stab Cat. No. #270
LowCross® HRP-Stab Animal-free/Protein-free Cat. No. #370
Sample Buffer Cat. No. #105, #106
Sample Buffer Animal-free/Protein-free Cat. No. #305
Covid-19 Inactivation and Dilution Buffer
SafetyTector™ S Cat. No. #400
Blockers
The Blocking Solution Cat. No. #110
BSA-Block Cat. No. #115
PlateBlock™ Cat. No. #112
SmartBlock™ Cat. No. #113
Stabilizers
Antibody Stabilizer TRIS Cat. No. #130
Antibody Stabilizer TRIS Animal-free/Protein-free Cat. No. #330
Antibody Stabilizer PBS Cat. No. #131
Antibody Stabilizer PBS Animal-free/Protein-free Cat. No. #331
AP-Protector® Cat. No. #235
Liquid Plate Sealer® Cat. No. #160
Liquid Plate Sealer® Animal-free Cat. No. #163
HRP-Protector™ Cat. No. #222
HRP-Protector™ Animal-free/Protein-free Cat. No. #322
Standard Buffers (ready-made)
Coating Buffer pH 7.4 Cat. No. #120
Coating Buffer pH 9.6 Cat. No. #121
Stripping Buffer Cat. No. #150
Washing Buffer PBS Cat. No. #140
Washing Buffer PBS IHC Cat. No. #141
Washing Buffer TRIS Cat. No. #145
Washing Buffer TRIS IHC Cat. No. #146
Publications
CoViD-19-antibody-test-challenge
Interference and HAMA problems
IVD technology article
Long-term stabilization of assay components
Optimisation of assays: Interference in immunoassays recognize and avoid
SARS-CoV-2 antigen tests: safety for less than 5 cents per test
Specificity, positive predictive value and validation statistics in the context of CoViD-19
The appropriate blocker — A short overview
The right strategy saves much costs
