Coating Buffer (10x Stock) Cat. No. #120 (TRIS), #121 (carbonate)

10x concentrate; Buffer for immobilization of proteins on surfaces. Coating Buffer for ELISA, EIA, RIA, protein arrays and immuno-PCR. Coating Buffer is designed for adsorptive immobilisation of proteins and antibodies on plastic surfaces.
£42.24

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Coating Buffer for ELISA, EIA, RIA, protein arrays and immuno-PCR.

Coating Buffer is designed for adsorptive immobilisation of proteins and antibodies on plastic surfaces (for example microtiterplates) or other protein binding surfaces. Depending on the surface as well as on proteins or antibodies the required incubation time can vary. Consequently, individual optimisation of the incubation procedure is recommended.

Depending on the protein or the antibody the pH of the Coating Buffer is crucial for efficient immobilisation. Select from Coating Buffer pH 7,4 (TRIS-based) and pH 9,6 (carbonate-based), as the pH-value can have an influence on the steric structure of proteins or antibodies, thus affecting their immobilisation.

For the optimisation of the coating procedure of a newly developed immunoasay we strongly recommend testing the two Coating Buffers side by side.
 

10x stock solution
Simply dilute with water!

Please note that the 1 L size will be packaged in 2x 500 ml bottles.
 


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Product Inserts and Data Sheets

Brand CANDOR Bioscience
Code 120 125

Product Flyers and Application Guides

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