Tools for better Covid-19 Antibody Tests
How CANDOR Bioscience products can help the development of better Covid-19 antibody tests:
Use the just launched novel PlateBlockTM to minimise the number of false positive results for antigen-down ELISAs detecting high-affinity anti-SARS-CoV-2 IgG antibodies in patient blood samples diluted as low as 1:10 to allow for excellent sensitivity in serological tests.
Combine low background with superior specificity for SARS-CoV-2 by using CANDOR's LowCross-Buffer® as anti-interference sample dilution buffer.
LowCross-Buffer® acts as an "affinity filter" that excludes assay signals due to low to medium affinity antibodies that may be already present against other related corona viruses from previous infections.
Instead, zoom in on human antibodies with high affinity to SARS-CoV-2 that are expected to confer Covid-19 immunity in asymptomatic individuals or those who experienced only mild Covid-19 symptoms.
The same approach is crucial for the assessment of immunity in healthy individuals currently taking part in clinical studies of experimental vaccine projects against SARS-CoV-2.
BIOAXXESS are pleased to offer CANDOR's ELISA Sampler Package CoViD-19 as a tool box, which contains the PlateBlockTM together with key CANDOR reagents required for professional ELISA development and manufacturing.
PlateBlock™ is an animal-free and protein-free blocker for plastic surfaces (e.g. microtiter plates). This blocker is optimized for serological assays (antigen-down-assays).
For this assay format, it is essential that the blocking does not cover the epitope of the capture antigen and that the detector antibody does not cross react with the blocking surface.
In addition, some serum and plasma specimens from patients tend to detach single molecules from the dense blocking layer and replace them with other molecules (mostly antibodies of the patients). This results in high background levels if the specimen is not highly diluted. (Antibodies in low concentration could not be detected if the dilution of the sample is too high). Additionally, cross-linking between blocking and detector antibodies can be initiated by serum proteins.
See Fig. 4 on PlateBlockTM compared to other ELISA plate blockers and Fig. 5 with an overview of an 'ideal' serological ELISA using CANDOR's professional assay solutions
(from CANDOR's White Paper 'Covid-19 Antibody Test Challenge')
From: £48.36PlateBlock™ is an animal-free and protein-free blocker for plastic surfaces (e.g. microtiter plates). This blocker is optimized for use with serological assays with surface-coated antigen.
£257.31ELISA Sampler package CoViD-19 is available based on TRIS (cat. no.: 600 030). The kit contains 125 ml LowCross-Buffer®, PlateBlock article, Coating Buffer pH 7.4 10x, Washing Buffer TRIS 10x, Liquid Plate Sealer® and LowCross® HRP-Stab.
£64.78After immobilisation of the antibodies/antigens and blocking, Liquid Plate Sealer® seals the plates with a stabilising layer with good solubility and doesn't affecting the assay afterwards.
£257.31ELISA Sampler package 2 CoViD-19 is available based on TRIS (cat. no.: 600 030). The kit contains 125 mL LowCross-Buffer® STRONG, 125 mL PlateBlock, 125 mL Coating Buffer pH 7.4 10x, 125 mL Washing Buffer TRIS 10x, 125 mL Liquid Plate Sealer® animal-free and 125 mL HRP-Protector™ article.