The Blocking Solution for ELISA, EIA, RIA, protein arrays, Western blotting, immuno-PCR and immunohistochemistry.
The most effective Blocker from CANDOR Bioscience
The Blocking Solution saturates free binding sites on plastic surfaces, membranes or other protein binding surfaces. The Blocking Solution prevents non-specific binding to surfaces.
By incubating the surface with The Blocking Solution (e.g. microtiter plate or western blotting membrane), free spaces on the surface are saturated by the components of The Blocking Solution. This minimizes unwanted binding of the analyte or the detection antibody to the surface and so background can be avoided and sensitivity of the assay can be improved.
The Blocking Solution is based on chemically modified and defined fragments of highly purified casein.
The Blocking Solution is not comparable to other commercially available or lab-made casein based solutions which are produced by just dissolving and filtrating of casein.
Benefits of The Blocking Solution
Generally speaking, the blocking effectiveness depends on different factors. The more effective the blocker, the more reliable are the results of the assays (even with different specimens). It is important to achieve complete saturation of the surface without any gaps. There should be no exchange reaction between molecules of the blocking layer and the sample matrix. In addition any interaction of molecules in the blocking layer with the sample matrix should also be prevented. A good blocker can fill even very small gaps and can minimize such interactions.
The Blocking Solution is the premium product of a new class of blockers developed by CANDOR Bioscience. These blockers carry chemical modifications which help to minimize interactions between the blocking layer and the sample matrix.
The Blocking Solution is based on highly purified casein which has been chemically modified. In addition the casein molecules were fragmented in an advanced production process. The specific procedure during fragmentation of the casein molecules results in a wide spectrum of variably sized casein fragments – reproducible from batch to batch.
Due to the variably sized casein fragments, the adsorption efficiency of classic protein-based blockers is combined with a blocking layer of small molecules and maximal density. The mixture of variably sized molecules ensures an extremely dense packing structure of the adsorptive blocking layer.
The chemical modification preserves these structures during the assay and minimizes the tendency to interactions. These advantages can be used without changing the blocking procedure. In many assays a reduction of incubation time for blocking can be realized by using The Blocking Solution.
The Blocking Solution is recommended for assays which require high reproducibility and low coefficients of variation. Examples are assays in medical and pharmaceutical research such as assays for pre-clinical and clinical studies, production of high quality diagnostics or measurement of precious specimens.
For research use only, not for diagnostic use.
Please note that the 1 L size will be packaged in 2x 500 ml bottles.
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Product Descriptions & Guides
Product Inserts and Data Sheets
| Brand | CANDOR Bioscience |
| Code | 110 125 |
Product Flyers and Application Guides
BIOAXXESS CANDOR Blocker Selection Guide NEW
BIOAXXESS CANDOR Product Application Guide
CANDOR Products Brochure
Assay Defender® Flyer NEW
Assay Defender® Use NEW
Assay Defender® LowCross-Buffer® Comparison Matrix NEW
SafetyTector™ S NEW
Product Inserts and Data Sheets
Assay Optimizers
Assay Defender® Cat. No. #180 NEW
LowCross-Buffer® Cat. No. #100, #101, #102
LowCross-Buffer® Animal-free/Protein-free Cat. No. #300, #301, #302
Low-Cross-HRP® Cat. No. #200
LowCross® HRP-Stab Cat. No. #270
LowCross® HRP-Stab Animal-free/Protein-free Cat. No. #370
Sample Buffer Cat. No. #105, #106
Sample Buffer Animal-free/Protein-free Cat. No. #305
Covid-19 Inactivation and Dilution Buffer
SafetyTector™ S Cat. No. #400 NEW
Blockers
The Blocking Solution Cat. No. #110
BSA-Block Cat. No. #115
PlateBlock™ Cat. No. #112 NEW
SmartBlock™ Cat. No. #113
Stabilizers
Antibody Stabilizer TRIS Cat. No. #130
Antibody Stabilizer TRIS Animal-free/Protein-free Cat. No. #330
Antibody Stabilizer PBS Cat. No. #131
Antibody Stabilizer PBS Animal-free/Protein-free Cat. No. #331
AP-Protector® Cat. No. #235
Liquid Plate Sealer® Cat. No. #160
Liquid Plate Sealer® Animal-free Cat. No. #163
HRP-Protector™ Cat. No. #222
HRP-Protector™ Animal-free/Protein-free Cat. No. #322
Standard Buffers (ready-made)
Coating Buffer pH 7.4 Cat. No. #120
Coating Buffer pH 9.6 Cat. No. #121
Stripping Buffer Cat. No. #150
Washing Buffer PBS Cat. No. #140
Washing Buffer PBS IHC Cat. No. #141
Washing Buffer TRIS Cat. No. #145
Washing Buffer TRIS IHC Cat. No. #146
All-in-One Western Blotting Solution
ReadyTector® Cat. No. #720 and 730
Tool boxes
Blocking Sampler Package with BSA Block Cat. No. #600
CANDOR Starter Package Cat. No. #600
CANDOR Starter Package IHC Cat. No. #600
Publications
CoViD-19-antibody-test-challenge
Interference and HAMA problems
IVD Technology Article
Long-term stabilization of assay components
Optimisation of assays: Interference in immunoassays recognize and avoid
SARS-CoV-2 antigen tests: safety for less than 5 cents per test
Specificity, positive predictive value and validation statistics in the context of CoViD-19
The Appropriate Blocker — A Short Overview
The right strategy saves much costs
